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Antibiotics (10,000 U/ml penicillin, 10 mg/ml streptomycin), PBS, and Trypsin EDTA solution C (0.05% trypsin0.02% EDTA) were obtained from Biological Industries (Kibbutz Beit Haemet, Israel), and fetal bovine serum (FBS) from Invitrogen (Carlsbad, CA, USA)

Chronic alcohol exposure has been shown to reduce BDNF expression, particularly in the hippocampus and prefrontal cortex, regions heavily implicated in mood regulation and cognitive function

As shown previously, the interaction with PMNs led to increased expression of PbSOD1 and PbSOD3 , both in the presenceto a greater extentand absence of DPI (S6 Fig)